［团队研究速递］一种制备简便、具有增强粘合强度的双交联粘合剂用于止血和感染伤口愈合（Biomaterials Advances，2025）

中英文摘要

摘要　尽管生物粘合剂在替代传统伤口缝合技术方面已展现出优势，但在伤口闭合、止血和愈合方面仍存在局限性，包括组织粘附力不足、潜在的生物毒性以及复杂的制备工艺。本研究开发了一种简便的制备可注射双交联多功能水凝胶粘合剂（Gel/EN/FBTA）的方法。Gel/EN/FBTA粘合剂是由单宁酸（TA）、3-甲酰基苯基硼酸（3-FPBA）和明胶组成的动态交联网络，可提供大量粘合位点并通过能量耗散增强粘合剂的内聚力。明胶内部的酰胺化反应可形成稳定的刚性交联，并稳定地维持粘合剂的结构。通过调节双交联网络的化学组成和交联密度，可以调节粘附力和内聚力之间的平衡。在此平衡条件下，Gel/EN/FBTA2水凝胶的粘附强度是商用纤维蛋白胶的3倍，在大鼠肝损伤、大鼠尾部损伤和兔肝十字切口模型中表现出良好的止血效果。此外，Gel/EN/FBTA2水凝胶粘合剂可有效治疗伤口感染，降低炎症水平，促进再上皮化，加速胶原沉积，实现感染性全层伤口的愈合。这种双网络设计范式为开发具有定制力学和生物活性特性的下一代生物粘合剂提供了一种通用策略，在不可压缩性出血和感染性伤口管理中显示出巨大潜力。

Abstract

Although biological adhesives have shown advantages in replacing traditional wound suturing techniques, there are still limitations in wound closure, hemostasis, and healing, including insufficient tissue adhesion, potential biological toxicity, and complex preparation processes. In this study, a facile route for preparing injectable dual-crosslinking multifunctional hydrogel adhesive (Gel/EN/FBTA) was developed. The Gel/EN/FBTA adhesive is a dynamic cross-linked network composed of tannic acid (TA), 3-formylphenylboronic acid (3-FPBA) and gelatin, which can provide a large number of bonding sites and strengthen the adhesive cohesion through energy dissipation. The amidation reaction inside gelatin can form stable rigid crosslinks and maintain the structure of the adhesive stably. The balance between adhesion and cohesion can be regulated by adjusting the chemical composition and crosslinking density of the dual-crosslinking network. Under this equilibrium condition, the adhesion strength of Gel/EN/FBTA2 hydrogel is 3 times that of commercial fibrin glue, which shows good hemostatic effects in rat liver injury, rat tail injury, and rabbit liver cross incision models. Furthermore, Gel/EN/FBTA2 hydrogel adhesive can effectively treat wound infection, reduce inflammation level, promote re-epithelialization, accelerate collagen deposition, and achieve the healing of infectious full-thickness wounds. This dual-network design paradigm provides a versatile strategy for developing next-generation bioadhesives with tailored mechanical and bioactive properties, demonstrating significant potential for non-compressible hemorrhage and infected wound management.

参考文献：

1. Ziyuan Guo, Zongliang Wang, Peibiao Zhang,A facilely prepared dual-crosslinking adhesive with enhanced adhesive strength for hemostasis and infected wound healing,Biomaterials Advances,Volume 176,2025,214349,ISSN 2772-9508,https://doi.org/10.1016/j.bioadv.2025.214349.(https://www.sciencedirect.com/science/article/pii/S2772950825001761)